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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes

Fig. 2

Generation of TRF3 knockout (TRF3−/−) hESCs. a Targeting strategy for the generation of TRF3−/− hESCs by homologous recombination using CRISPR/Cas9 technology. b Translation analysis of TRF3 truncated proteins in TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a1/2, the TRF3 genome sequence of each allele in TRF3−/−-2 hESCs. c Potential off-target sites predicted by CHOPCHOP. The predicted off-target sites are in the “Name of putative sites” column. The mismatched bps are shown in red. The protospacer adjacent motif is shown in cyan. Indels: the confirmed mutant bps at each potential off-target site

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Stem Cell Research & Therapy

ISSN: 1757-6512