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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes

Fig. 3

The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT-PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

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Stem Cell Research & Therapy

ISSN: 1757-6512