Fig. 1
From: Role of CD133 in human embryonic stem cell proliferation and teratoma formation
CD133 is highly expressed in human embryonic stem cells. a Analysis of CD133 mRNA expression levels of two human embryonic stem cell (WA26 and RuES2, passage 20), HCT116, U2OS, HeLa, and HEF by RT-qPCR. Two paired primers were used. Bars indicate mean ± SD (n ≥ 2). b The protein level of CD133 in human cell lines determined by Western blot (immunoblotting) using a commercial CD133 antibody (Biorbyt, orb10288). c Flow cytometry analysis of these human cells using a commercial anti-CD133/1 (AC133)-APC conjugated antibody (Miltenyi Biotec, 130-098-129). The bar plot shows the percentage of CD133-positive cell in each cell line