Fig. 9

Western blot analysis of TLR4/NF-κB signaling pathway-related molecules. A Expression of key molecules involved in the TLR4/NF-κB pathway as detected by western blotting. B Relative gray value quantitative evaluation of TLR4/NF-κB pathway-related molecules. On POD 0 (naive), the expression of molecules upstream of TLR4 was not significantly changed, but that of the downstream molecules (p-IκBα and p-p65) was altered. The expression of downstream molecules in the SCS group was higher than that in NMP, BP, and HBP groups (P < 0.05), but no significant difference was observed between these three groups. On POD 1, the expression of MYD88, TRAF6, p-IκBα, and p-p65 in the BP and HBP groups was lower than that in the SCS and NMP groups (P < 0.05), but no difference in expression was observed between the BP and HBP groups. On POD 7 and 14, the expression of TLR4 pathway-related molecules (MYD88, TRAF6, p-IκBα, and p-p65) in the HBP and BP groups was significantly lower than that in the SCS and NMP groups, and its expression in HBP group was lower than that in BP group (P < 0.05). (^aP < 0.05 vs. SCS group; ^bP < 0.05 vs. NMP group; ^cP < 0.05 vs. HBP group)