Fig. 7

PMVs restored cellular physiology in APAP-treated HepG2 cells. HepG2 were treated with 90 mM APAP for 3 h. After washing, cells were harvested and re-seeded in 96-wells precoated with ECM (extracellular matrix) prepared from HTB9 cells. Mito-Tempo (MT) or PMVs from hUCMSCs (with VSV-G) were added at 4 h. PBS was used as the negative control. Intracellular levels of AST (a), ALT (b), total (T-) GSH (c), and GSSG (d) were measured using respective colorimetric approaches. GSH (e) and the ratio of GSH to GSSG (f) were calculated as described in the “Materials and methods” section. The values are shown as mean ± SEM (n = 3 experiments). Bars sharing a letter in common are not significantly different (P > 0.05) (one-way ANOVA Student’s t test)