Fig. 3

In vivo therapeutic effects of iHep cells in acute liver fibrosis. a A schematic diagram of the procedure for the in vivo study. b A representative picture of the damaged liver after iHep cell injection. The shrunken liver, irregular surface, and damaged fibrotic areas of the liver in the PBS- or iPS-treated mice were observed. c Representative pictures of H&E staining 3 weeks after cell transplantation. Bar = 100 μm. d Quantitative analysis of collagen content from liver tissues. The hepatic collagen content was measured using Sirius red/fast green collagen staining. Sectioned liver tissues were fixed in Kahle’s fixative and stained with dye solution and mixed with a dye extraction buffer. The O.D. values of dye solutions were read by a spectrophotometer. n = 7 per group. **p < 0.01; *p < 0.05. e Representative pictures of fibrosis by Masson’s trichrome (M-T) staining after cell transplantation. Bars = 100 μm. f Quantitative analysis of the fibrotic area by M-T staining. **p < 0.01; *p < 0.05, n = 10 per group