Fig. 2

Experiments in the presence of high glucose DMEM (25 mM). a, b Effect of sucralose on reactive oxygen species (ROS) accumulation has been tested in vitro by Mitosox staining (n = 3). Accumulation of ROS in human mesenchymal stromal cells (MSCs) increased secondary to sucralose exposure in a dose-dependent manner. Response of ROS accumulation in the presence of sucralose appears to be accentuated in the presence of higher levels of glucose in the cell media. c Viability of cells (N = 2) when exposed to sucralose in a dose-dependent manner in the presence of high glucose showed decreasing cell viability in increasing concentrations. d Effects on antioxidants and adipogenic genes in a dose-dependent manner when MSCs were exposed to sucralose in the presence of high glucose. Statistical analysis: unpaired t test was performed by considering HG (high glucose) as control (N = 2 for qPCR)