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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Fig. 6

a Expression levels of miR-129-5p during the osteoblast differentiation process of ADSCs, as detected by RT-PCR. b Binding site between miR-129-5p and the wt/mut LINC00314 3′UTR. c Luciferase activity (LINC00314 3′UTR wt and LINC00314 3′UTR mut) in agomiR-NC- and agomiR-miR-129-5p-transfected cells. d Relative expression of miR-129-5p in the agomiR-NC, agomiR-miR-129-5p, agomiR-miR-129-5p+pcDNA3.1-LINC00314, antagomiR-NC, antagomiR-miR-129-5p, and antagomiR-miR-129-5p+sh-LINC00314 groups. e Relative expression of Runx2, Osterix, and osteocalcin after transfection. f Western blot analysis of Runx2, Osterix, and osteocalcin after transfection. g ARS and ALP staining in the transfection groups. **P < 0.01, compared with the NC group; ##P < 0.01, compared with the sh-NC group

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