Fig. 6From: Melatonin-stimulated MSC-derived exosomes improve diabetic wound healing through regulating macrophage M1 and M2 polarization by targeting the PTEN/AKT pathwayMT-Exo suppressed inflammation by activating the PTEN/AKT signalling pathway. a The expressions of PTEN, phosphorylation of AKT, AKT (total AKT) and GAPDH were tested in RAW264.7 cells after treatment with PBS, LPS (100 ng/mL), LPS + Exo and LPS + MT-Exo for 24 h by Western blotting. GAPDH was utilized as an internal reference. b The expressions of PTEN, phosphorylation of AKT, AKT (total AKT) and GAPDH were tested in vivo after treatment with PBS, Exo and MT-Exo by Western blotting. GAPDH was utilized as an internal reference. c The quantification of the greyscale values of PTEN/ GAPDH and p-AKT/AKT in vitro (n = 3, *p < 0.05). d The quantification of PTEN/ GAPDH and p-AKT/ AKT by Western blotting in vivo (n = 3, *p < 0.05)Back to article page