Fig. 2

Neural cell populations. Representation of neural populations in BS-SC (A–P) sections and SC (A′–P′) whole slices at in situ (from 8.5 to 10.5 w.) and 21 DIV. Expression of nestin (A,B,A′,B′), DCX (C,D,C′,D′ from 5.5 w. case), MAP-2 (E,F,E′,F′), NF (G,H,G′,H′), TH (I, J, I′, J′), 5-HT (K,L,K′,L′), GFAP (M, N, M′, N′), Iba1 (O,P,O′P′). Hoechst (blue) dye was used to stain cell nuclei. Bar: 50 μm in I–L and I′–L′; 100 μm in the others. A–M, O–A′, C′, E′, G′, I′, K′, M′, O′: confocal micrograph from 10 μm section; N, B′, D′, F′, H′, J′, L’, N′, P′: confocal micrograph from whole slice. Abbreviations: BS-SC, longitudinal brain stem-spinal cord slices; SC, spinal cord cross-sectional slices; VF, ventral funiculi; DH, dorsal horns; VH, ventral horns