Fig. 4

Calcium signaling A (a) Representative image of a SC hOC slice (7 DIV) loaded with the Ca²⁺ indicator OGBPT-1. (b) Ca²⁺ traces of three individual cells (shown in a) under basal conditions (before stimulation). (c) Ca²⁺ traces of the same three cells after stimulation with 100 μM ATP (black thick line). (d) Representative image of a SC hOC slice (21 DIV) loaded with the Ca²⁺ indicator OGBT-1. (e) Ca²⁺ traces of three individual cells (shown in d) under basal conditions (before stimulation). (f) Ca²⁺ traces of the same three cells after stimulation with 100 μM ATP (black thick line). B Graph showing the percentage of cells with spontaneous Ca²⁺ activity and evoked Ca²⁺ responses after ATP stimulation in SC hOC slices at 7 DIV and 21 DIV. Values are expressed as mean + SEM. Abbreviations: DIV, days in vitro; OGBPT-1, Oregon Green Bapta-1