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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human ex vivo spinal cord slice culture as a useful model of neural development, lesion, and allogeneic neural cell therapy

Fig. 5

Electrophysiological recordings: Representative stimulus-evoked potentials obtained in longitudinal hOCs derived from 4.5 and 6 w cases and recorded after 7, 14, and 15 DIV. A The stimulation electrode and the extracellular recording pipette were placed at distances between 500 and 800 μm along the rostro-caudal axis. Potentials were evoked by single pulse stimulation (50 μs; 0.03 Hz). B Input-output traces in the left panel were obtained by increasing the stimulation strength from 200 μA to 2 mA. Preventing neuronal depolarization with 1 μM TTX abolishes stimulus-evoked potentials (gray traces), note that after 30 min wash-out, responses were completely restored (superimposed black trace). Abbreviations: DIV, days in vitro; TTX, tetrodotoxin

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Stem Cell Research & Therapy

ISSN: 1757-6512