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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Knockdown of LAP2α inhibits osteogenic differentiation of human adipose-derived stem cells by activating NF-κB

Fig. 1

LAP2α is upregulated in hASCs upon osteogenic differentiation. a Relative mRNA expression of LAP2α and the osteogenic markers RUNX2, ALP, and OCN, as measured by qRT-PCR during osteogenic differentiation of hASCs. GAPDH was used for normalization, n = 6. b Western blotting and quantification of protein levels of LAP2α, RUNX2, and the internal control GAPDH during osteogenic differentiation of hASCs, n = 3. c Western blotting and quantification of protein levels of LAP2α in hASCs, hBMMSCs, osteosarcoma cell lines U20S, 293T, and human gingival fibroblasts (hGF), n = 3. Data are shown as the mean ± SD; *P < 0.05 compared with the control group; **P < 0.01 compared with the control group; NS: not significant

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