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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Nonadherent culture method promotes MSC-mediated vascularization in myocardial infarction via miR-519d/VEGFA pathway

Fig. 6

The secretion of VEGFA in VEGFA-overexpressing nonAC-hMSCs attenuates miR-519d-mediated inhibition of tube formation of HUVECs. a The expression and secretion of VEGFA in the nonAC-hMSCs co-transfected with miR-519d mimics and VEGFA plasmid were measured using RT-qPCR, b western blotting, and c ELISA, respectively. d Tube formation assay was performed to detect the capillary-like tubules formation of HUVECs cultured in the presence of hMSC-conditioned medium. Data are represented as mean ± SD (n = 3 per group). P < 0.05 compared to miR-Ctrl group. #P < 0.05 compared to miR-519d group

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