Fig. 6

hUMSCs co-culture with TICs inhibit CDDP-induced autophagy in TICs through the AMPK/mTOR signaling pathway. a Protein expression of AMPK, mTOR and LC3B following 24 h of hUMSCs co-culture with TICs with or without Compound C or 3MA as determined using western blot analysis. GAPDH was used as an internal control to quantify protein amounts. b–d Quantification of phosphorylated AMPK, mTOR and LC3B expression levels within each group. e, f Analysis of ratios of RNA levels for Ampk/Gapdh and Mtor/Gapdh within each group. Data are expressed as the means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. hUMSCs, human umbilical cord-derived mesenchymal stem cells; TICs, theca-interstitial cells; CDDP, cisplatin; AMPK, Amp-activated protein kinase; mTOR, mammalian target of rapamycin; 3MA, 3-methyladenine