Fig. 5
From: Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
Inhibition of HDAC9 partially restores bone loss in aged mice. a Micro-CT analysis of trabecular bone mass and cortical bone thickness in the femora of aged mice from the control, shScr-treated, and shHDAC9-treated groups was examined 4 weeks and 8 weeks operation, respectively. Quantitative analysis of bone mineral density (BMD), trabecular bone volume (BV/TV), and cortical bone thickness (Ct.Th) was performed. Scale bar = 1 mm. b SAbetaGal staining was performed in the bone marrow of mice from the control, shScr-treated, and shHDAC9-treated groups 4 weeks after treatment, and SAbetaGal positive signals were quantitatively analyzed. Scale bar = 100 μm. c Immunofluorescent staining of OCN and PPAR-γ was performed in the femora 4 weeks after treatment, and quantitative relative positive ratio areas of the femora were analyzed. Scale bars = 100 μm. d Immunofluorescent staining of aggrecan and collagen II was performed in the femora 4 weeks after administration, and quantitative relative positive ratio areas of the femora were analyzed. Scale bars = 50 μm. n = 7 mice per group. The data are analyzed with one-way analysis of variance (ANOVA) and presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001