Fig. 2
Prenatal dexamethasone exposure (PDE) suppresses postnatal long bone development in female mouse offspring. a Representative images of hematoxylin-eosin (H&E) staining, Goldner’s trychrome staining, and tartrate-resistant acid phosphatase (TRAP) staining in femoral sections from 2-, 4-, and 6-week-old female mouse offspring. 2W, 4W, and 6W represent 2-, 4-, and 6-week-old mice, respectively. Scale bar, 100 μm. Quantitative analysis of the numbers of osteoblast per tissue area in metaphyseal bone below growth plate (N. Ob per mm2) (b) and the numbers of TRAP+ cells per tissue area in metaphyseal bone below growth plate (N. TRAP+ cells per mm2) (c). Data are represented as mean ± S.E.M. *P < 0.05 versus control (n = 5 per group, Student’s t test)