Fig. 6

Clearance of senescent cell by treatment with senolytics dasatinib and quercetin (D+Q) rescues the loss of osteoprogenitors and BMSCs in female mouse offspring after PDE. a Representative images of the flow cytometry analysis and b the percentage of Nestin⁺ cells in femoral bone from 4-week-old female mouse offspring. Pregnant mice were injected subcutaneously with normal saline (control) or dexamethasone (1.2 mg/kg/day) (PDE group) during gestational days (GD) 12–14. Additionally, control pregnant mice were treated with 1% methyl cellulose, and PDE pregnant mice were treated with 1% methyl cellulose (vehicle) or dasatinib (5 mg/kg/day) and quercetin (50 mg/kg/day) by oral gavage during GD 12–14. Femoral bone marrow of 4-week-old female mouse offspring was harvested for analysis. c Representative images of the flow cytometry analysis and b the percentage of CD45⁻CD29⁺CD105⁺Sca-1⁺ cells in femoral bone from 4-week old female mouse offspring. n = 5 per group. *P < 0.05, one-way analysis of variance (ANOVA) with the Bonferroni post hoc test