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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013

Fig. 3

DLX5 associated with HOXC8 and formed protein complex in SCAPs. a Co-IP results showed more DLX5-HOXC8 complex formation in DLX5 over-expressed SCAPs b Co-IP results showed less DLX5-HOXC8 complex formation in DLX5-silenced SCAPs. c Real-time RT-PCR results showed HOXC8 over-expression in SCAPs. d Western blot results showed HOXC8 over-expression in SCAPs. Co-IP results showed more HOXC8-DLX5 complex formation in HOXC8 over-expressed SCAPs. e Real-time RT-PCR results show HOXC8 depletion in SCAPs. f Western blot results show HOXC8 depletion in SCAPs. Co-IP results showed fewer endogenous HOXC8-DLX5 protein complexes in HOXC8-silenced SCAPs. g HOXC8 expression was increased during the chondrogenic differentiation in SCAPs according to real-time RT-PCR results. GAPDH served as the internal control. One-way ANOVA or Student’s t test analysis was performed to determine the statistical significance. All error bars represent s.d. (n = 3). *P ≤ 0.05. ** P ≤ 0.01. Consh, control shRNA; DLX5sh, DLX5 shRNA; HOXC8sh, HOXC8 shRNA

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Stem Cell Research & Therapy

ISSN: 1757-6512