Fig. 1

Characterization of the hiPSC. a hiPSC expressed high levels of the pluoripotency marker genes, OCT3/4, SOX2, and NANOG, which were comparable to hESC, a human embryonic stem cell (t test; NS, not significant; ND, not detectable; n = 3). b Ki67, pluoripotency markers of hiPSC were identified using IF method. c–e Pluoripotency markers of hiPSC (NANOG, OCT4, SSEA4, and TRA-1-60) were confirmed by immunoflorescence (IF) assay. g, h Representative flow cytometry analysis further confirmed the pluoripotency of the hiPSC. Scale bars, 100 μm (× 400). Expression values of all PCR analyses were normalized to the housekeeping gene GAPDH. Data are presented as “mean ± SD”