Fig. 2
Rapamycin alleviates stress-induced senescence of hUC-MSCs. 2 × 105 hUC-MSCs (p8-p9) were seeded in 6-well plates and treated with Dox or H2O2 with or without rapamycin. a Cells were collected 4 days after Dox or H2O2 treatment. β-Gal staining showing that rapamycin (5 μM) alleviates Dox- or H2O2-induced hUC-MSC senescence (scale bar = 100 μm). The quantification data is shown on the right. Data are presented as the mean ± SEM. **p < 0.01;***p < 0.001 by one-way ANOVA with Tukey’s post hoc test. b Cells were collected 2 days after Dox or H2O2 treatment. qRT-PCR analysis shows rapamycin reduces H2O2-induced SASP. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01;***p < 0.001 by one-way ANOVA with Tukey’s post hoc test. c Representative Western blot shows that rapamycin reduces the activation of p70S6K and alleviates stress-induced DNA damage response. Quantification data is shown on the right. Data are achieved from three independent experiments. *p < 0.05; **p < 0.01;***p < 0.001 by two-way ANOVA with Bonferroni post hoc test first, followed by paired Student t test