Fig. 4

Differentiation evaluation of CM-Dil-labeled hUC-MSCs. The target protein color was green, the DAPI with Dil-hUC color was magenta representing the living cells, and the superposition color of green and magenta was white which means differentiated cells (arrow). CK7 was expressed in the cytoplasm of epithelial cells, CD31 was expressed in the cytoplasm of vascular endothelial cells, and ERα was expressed in the nucleus and cytoplasm. From the 2nd week of treatment, hUC-MSCs began to differentiate into epithelial cells, vascular endothelial cells, and a small amount of ER cells