Fig. 5
From: Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
Involvement of vitamin D receptor in ASC antibacterial activity. ASCs cultured in antibiotic-free medium were replated in tissue culture-treated 6-well plates (0.5 × 105 cells/2 mL/well) and treated for 1 day with the following: (1) TLR agonists, MPLA (12.5 μg/mL); TLR 4 antagonist, CLI-095 (2.5 μM), or TLR 2/4 antagonist, OXPAPC (25 μM) for 1 day; or (2) vitamin D receptor inhibitor, GW0742 (12.5 μM), with or without 1,25(OH)2D3 (100 nM). Antibacterial activity was assayed as described in the “Materials and methods.” The results showed that treatment with CLI-095, OxPAPC, and MPLA did not affect the antibacterial activity of ASCs. On the other hand, treatment with the vitamin D signaling inhibitor, GW0742, almost completely suppressed the antibacterial activity of 1,25(OH)2D3 activated and unactivated ASCs. Results are derived from 3 to 5 patients (n = 7 to 14) and expressed as mean ± SD. VD3: 1,25(OH)2D3.