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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Osteocyte-derived exosomes induced by mechanical strain promote human periodontal ligament stem cell proliferation and osteogenic differentiation via the miR-181b-5p/PTEN/AKT signaling pathway

Fig. 5

PTEN is a target gene of miR-181b-5p. a The levels of miR-181b-5p in Exosome and Exosome-MS were detected using real-time PCR. b Cell localization of miR-181b-5p in HPDLSCs treated with exosomes according to FISH (scale bar 100 μm). c The number of target genes of miR-181b-5p predicted by the PITA, microT, TargetScan, and miRnada databases. In total, 157 crossover genes were obtained. d The binding site between miR-181b-5p and PTEN. e Real-time PCR showed PTEN level when HPDLSCs were transfected with miR-181b-5p mimics. f real-time PCR showed PTEN level when HPDLSCs were transfected with miR-181b-5p inhibitor. g Fluorescence intensity was calculated by ImageJ. h Enrichment information for biotin-labeled miR-181b-5p binding to PTEN (*P < 0.05, **P < 0.01, and ***P < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512