Fig. 3

miR-203’s depression on Wnt and Notch causes weak ESCs proliferation. Expression of Wnt and Notch signaling pathway in rat with diabetes mellitus by qRT-PCR (a). Expressions of Wnt’s and Notch’s downstream in rat with diabetes mellitus by qRT-PCR (b). Expression of Wnt and Notch signaling pathway in ESCs co-cultured with high glucose by qRT-PCR (c). Expression of Wnt and Notch signaling pathway in ESCs co-cultured with high glucose by WB (d). Expression of Wnt’s and Notch’s downstream in ESCs co-cultured with high glucose by qRT-PCR (e). Expression of Wnt and Notch signaling pathway in miR-203a-overexpressed ESCs by qRT-PCR (f). Expression of Wnt and Notch signaling pathway in miR-203a-overexpressed ESCs by WB (g). Expressions of Wnt’s and Notch’s downstream in miR-203a-overexpressed ESCs by qRT-PCR (h). Data are representative profile of clustered miRNA in individual samples and expressed as the mean ± SD of each group of samples from at least three separate experiments. *p < 0.05; **p < 0.01; ***p < 0.001