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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: miR-200a contributes to the migration of BMSCs induced by the secretions of E. faecalis via FOXJ1/NFκB/MMPs axis

Fig. 1

Enterococcus faecalis supernatants (EfS) increased the migration of rat bone marrow mesenchymal stem cells (BMSCs). a Harvesting of Enterococcus faecalis ATCC33186 cell-free culture supernatants. Enterococcus faecalis ATCC33186 was cultured in brain heart infusion (BHI) medium and culture supernatants were harvested at late stationary phase. The growth was recorded with the optical density of medium over time. b Observation of BMSCs migration with the stimulation of Enterococcus faecalis supernatants (EfS) in transwell assays. BMSCs were cultured on the upper inserts of transwell in DMEM with EfS. Those cells migrated to the lower side of transwell membrane were fixed, stained with crystal violet, and counted. c Observation of BMSCs migration with the stimulation of EfS in scratch wound assays. The quantitative results are the means ± SD of three independent experiments. *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512