Fig. 4

UC-MSCs inhibited the differentiation and enhanced the suppressive activity of MDSCs and the subsets in NOD mice. Bone marrow cells were cultured in RPMI-1640 with 40 ng/ml IL-6 and GM-CSF and with or without UC-MSC-conditioned medium (MSC-CM) for 4 days. a, c Representative flow cytometry results and percentage of b MDSCs, d PMN-MDSCs, and e M-MDSCs. f qPCR results of MDSC suppressive activity genes Arg-1, gp91phox, iNOS, TGF-β1, and IL-1β. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n = 4