Table 1 Primers for inflammatory cytokines used in the real-time quantitative PCR assay
Gene | Primer sequence | |
|---|---|---|
IL-1β | Forward | CCCTGAACTCAACTGTGAAATAGCA |
Reverse | CCCAAGTCAAGGGCTTGGAA | |
IL-6 | Forward | ATTGTATGAACAGCGATGATGCAC |
Reverse | CCAGGTAGAAACGGAACTCCAGA | |
TNF-É‘ | Forward | TCAGTTCCATGGCCCAGAC |
Reverse | GTTGTCTTTGAGATCCATGCCATT | |
IL-4 | Forward | CAAGGAACACCACGGAGAAC |
Reverse | CTCAGTGAGTTCAGACCGCT | |
TGF-β | Forward | GAAGTCACCCGCGTGCTAATGG |
Reverse | GTGTGTCCAGGCTCCAAATGTAGG | |
IL-10 | Forward | GTGTGTCCAGGCTCCAAATGTAGG |
Reverse | CAAGGCTTGGCAACCCAAGTA | |
β-Actin | Forward | CCTCTTTGCATGTCTCACTC |
Reverse | AATGTCACGCACGATTTCC | |