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Table 1 The constructed sequences used in this study

From: MicroRNA-503-3p affects osteogenic differentiation of human adipose-derived stem cells by regulation of Wnt2 and Wnt7b under cyclic strain

Genes

Sequence

miR-503-3p mimic

 

5′-GGGGUAUUGUUUCCGCUGCCAGGdTdT-3′

miR-Ctrl mimic

 

5′-UUGUACUACACAAAAGUACUGdTdT-3′

miR-503-3p inhibitor

 

5′-CCUGGCAGCGGAAACAAUACCCCdTdT-3′

miR-Ctrl inhibitor

 

5′-CAGUACUUUUGUGUAGUACAAdTdT-3′

siWnt2 siWnt7b

Sense

5′-GCCUUUGUUUAUGCCAUCUdTdT-3′

Antisense

5′-AGAUGGCAUAAACAAAGGCdTdT-3′

Sense

5′-CAGACCUGGUGUACAUUGAdTdT-3′

Antisense

5′-UCAAUGUACACCAGGUCUGdTdT-3′

siR-Ctrl

Sense

5′-UUCUCCGAACGUGUCACGUdTdT-3′

Antisense

5-ACGUGACACGUUCGGAGAAdTdT-3