Fig. 1

Generation of GFP tagged mouse bone marrow-derived mesenchymal stem cells and differentiation into functional pseudo-islets. a Schematic representation for BMSC isolation and stable GFP+ clone selection. b Fluorescent images of stable GFP⁺ (green) expressing BMSC and flow-cytometric quantification of sorted GFP⁺BMSC line. c Immunophenotyping of mesenchymal stem cell markers in GFP⁺BMSC in comparison untransfected BMSC using flow cytometry. d Schematic representation of islet differentiation protocol into functional islet-like cell clusters and representative microscopic images of GFP⁺BMSC at days 0, 4, and 7. Immunostaining images for vimentin (red) and insulin (green) are represented at initiation and completion of differentiation steps. e Immunostaining images for insulin (green) and somatostatin (red); c-peptide (green) and glucagon (red); NeuroD1 (green) and Neurog3 (red), and pdx1 (red) and Nestin (green) in differentiation islet-like clusters