Direct lineage tracing reveals Activin-a potential for improved pancreatic homing of bone marrow mesenchymal stem cells and efficient ß-cell regeneration in vivo

Table 1 List of primary antibodies

Sr. no.	Antibody	Company and catalog no.	Isotype IgG	Mono/polyclonal Ab	Mol. weight (KDa)	Application	Dilution
1	Nestin	Sigma#N5413	Rabbit	Poly	177	Western	1:1000
2	Pdx-1	BD#554655	Mouse	Mono	40	Western/IF	1:1000/1:200
3	Neurogenin-3	Sigma #SAB1306585	Rabbit	Poly	23	Western/IF	1:1000
4	β-Actin	BD#612657	Mouse	Mono	42	Western	1:10000
5	Nestin-PE	BD#561230	Mouse	Mono	177	IF	1:100
6	Insulin	CST#4590	Rabbit	Poly	6	IF	1:100
7	Glucagon	Sigma#G 2654	Mouse	Mono	3.48	IF	1:100
8	Somatostatin	Sigma #SAB4502861	Mouse	Poly	12	IF	1:100
9	CD90.2-FITC	BD#55302	Rat	Mono	~ 20	Flowcytometry/IF	1:10/1:500
10	CD44-PE	BD#553134	Rat	Mono	82	Flowcytometry/IF	1:10/1:500
11	CD34-FITC	BD#553733	Rat	Mono		Flowcytometry	1:10
12	CD45-APC	BD#559864	Rat	Mono		Flow cytometry	1:10
13	CD117-PE	BD#553869	Rat	Mono		Flowcytometry	1:10
14	CD49b	BD#554999	Rat	Mono		Flowcytometry	1:10
15	Vimentin	Sigma#C9080	Mouse	Mono	53	IF	1:400
16	Smooth muscle actin	Sigma#F3777	Mouse	Mono	42	IF	1:250
17	C-Peptide	CST#4593	Rabbit	Mono	5	IF	1:100

ISSN: 1757-6512