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Table 1 List of primary antibodies

From: Direct lineage tracing reveals Activin-a potential for improved pancreatic homing of bone marrow mesenchymal stem cells and efficient ß-cell regeneration in vivo

Sr. no. Antibody Company and catalog no. Isotype IgG Mono/polyclonal Ab Mol. weight (KDa) Application Dilution
1 Nestin Sigma#N5413 Rabbit Poly 177 Western 1:1000
2 Pdx-1 BD#554655 Mouse Mono 40 Western/IF 1:1000/1:200
3 Neurogenin-3 Sigma #SAB1306585 Rabbit Poly 23 Western/IF 1:1000
4 β-Actin BD#612657 Mouse Mono 42 Western 1:10000
5 Nestin-PE BD#561230 Mouse Mono 177 IF 1:100
6 Insulin CST#4590 Rabbit Poly 6 IF 1:100
7 Glucagon Sigma#G 2654 Mouse Mono 3.48 IF 1:100
8 Somatostatin Sigma #SAB4502861 Mouse Poly 12 IF 1:100
9 CD90.2-FITC BD#55302 Rat Mono ~ 20 Flowcytometry/IF 1:10/1:500
10 CD44-PE BD#553134 Rat Mono 82 Flowcytometry/IF 1:10/1:500
11 CD34-FITC BD#553733 Rat Mono   Flowcytometry 1:10
12 CD45-APC BD#559864 Rat Mono   Flow cytometry 1:10
13 CD117-PE BD#553869 Rat Mono   Flowcytometry 1:10
14 CD49b BD#554999 Rat Mono   Flowcytometry 1:10
15 Vimentin Sigma#C9080 Mouse Mono 53 IF 1:400
16 Smooth muscle actin Sigma#F3777 Mouse Mono 42 IF 1:250
17 C-Peptide CST#4593 Rabbit Mono 5 IF 1:100