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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey

Fig. 4

Generation and characterization of HLA-A knockout iPSCs. a Clone morphology after electroporation. b DNA sequencing results of knockout cell lines showed the double peak phenomenon. c DNA sequencing results showed the deletion loci. d Western blot analysis revealed HLA-A knocked out in iPSCs. e HLA-A−/−-iPSCs had alkaline phosphatase activity and were positive for Oct3/4, Sox2, and SSEA4

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