Fig. 5

Experimental validation of novel exon identified in Specc1l gene. a IGV snapshot of the novel (unannotated) exon identified in Specc1l gene illustrating read coverage in all 3 replicates of both TSCs and TGCs cells. Read coverage for novel exon is enclosed by a red box. Reference gene track is shown at the bottom (gray line) with bars representing the corresponding exons and arrowhead (black) showing the orientation of the gene. b Primer design strategy used for the amplification of novel exon. Forward primer (top arrow) was designed in a known exon (dark gray rectangle) while reverse primer (bottom arrow) was designed in a novel exon (black rectangle). The two exons are separated by the intronic region (black line). c PCR amplification of the novel exon from TSCs and TGCs. Agarose gel image of the PCR-amplified products of a novel exon in Specc1l gene. Amplification of actin (Actb) from RT+ and RT− (with and without reverse transcriptase) represents positive and negative controls, respectively.