Fig. 5

Maturation state of erythropoietic cells in the spleen and in the BM of the groups tested. a Flow cytometry of the different stages of erythropoiesis in the BM and spleen in mouse groups as tested with cell-markers CD71 and Ter119 [27]. EryA cells are less mature, large proliferating erythropoietic progenitors, both CD71⁺ and TER119⁺. EryB cells are smaller CD71⁺ cells. EryC representative is most mature small non dividing erythrocytes. Distribution of these cell populations harvested from the BM and spleen at the termination of the follow-up is presented in b. A comparison of Ter119 Cy3 nucleated cells staining of liver cryosections of non-operated and |Spl-] f-hPSC-treated TBI mice (c). Only a few individual cells (marked by a circle) where stained in the liver sinusoids, with no apparent liver extramedullary erythropoiesis, in spite of the higher hematopoietic stress in [Spl-] group due to the absence of spleen-EMH (p values: *> 0.05, **> 0.01, ***> 0.005)