Fig. 7

Hypoxia-preconditioned HUVECs were cocultured with MSCs to promote osteogenesis and angiogenesis. a MSCs were cultured in alone or were cocultured with HUVECs or with hypoxia-preconditioned HUVECs (HPC HUVECs). The OIM was changed every 3 days, NBT/BCIP staining was performed to detect ALP enzyme activity on day 7, and alizarin red staining was performed to detect calcium deposition on days 14 and 21. Scale bar, 100 μm. b A Matrigel tube assay was performed to test the angiogenic ability of HUVECs. Scale bar, 100 μm