Fig. 2

Matrilin-3 priming and culture condition optimization. a Live/dead assay. b mRNA expression of the apoptosis markers p53 and BAX. c Human cytokine array analysis was performed to measure the growth factors and cytokines in the conditioned medium of Ad-MSCs and primed Ad-MSCs. d Expression of TGF-β1, TGF-β2, IL-10, GCSF, and MMP-1 levels normalized to positive control. e Expression of CCL-5, GDF-15, and IL-6 levels normalized to positive control. G1, Ad-MSC monolayer; G2, matrilin-3-primed Ad-MSC monolayer; G3, Ad-MSC spheroid (125 cells/microwell); G4, matrilin-3-primed Ad-MSC spheroid (125 cells/microwell); G5, matrilin-3-primed Ad-MSC spheroid (250 cells/microwell); G6, matrilin-3-primed Ad-MSC spheroid (500 cells/microwell). Statistically significant expression at *p < 0.05, **p < 0.01, ***p < 0.001