Fig. 2

Multi-lineage differentiation of BMSCs and ASCs in HPL. a Osteogenic differentiation: relative expression (fold changes) of early, intermediate, and late osteogenic gene markers in BMSCs and ASCs after 7 days of induction. Data represent means; each symbol represents a single donor (n = ≥ 3 donors) based on the average of ≥ 2 experimental replicates; statistical analyses are based on delta-Ct values; *p < 0.05; **p < 0.001. b ALP activity in BMSCs and ASCs after 7 and 14 days of osteogenic induction. Data represent means ± SD (n = 3 donors); **p < 0.001. Representative images of Alizarin Red S (ARS) staining (c) and quantification (d) after 21 days. e Adipogenic differentiation: representative images of Oil red O (ORO) staining and quantification (f) after 14 days. Scale bars 100 μm. Data represent means ± SD (n = 3 donors); **p < 0.001