Fig. 2

DNMT1 promotes adipogenic differentiation of HFSc. a Oil red O staining was used to detect the adipogenic differentiation of HFSc. b Western blot analysis detected the expression of DNMT1 normalized to GAPDH in HFSc cultured in differentiation medium for 0 days, 7 days, and 14 days. c Western blot analysis detected the expression level of DNMT1 by overexpression of DNMT1 normalized to GAPDH. d The degree of adipogenic differentiation of HFSc after overexpression of DNMT1 was detected by oil red O staining. e Western blot analysis was used to detect the expression level of adipose formation markers normalized to GAPDH. Data were expressed as mean ± standard deviation. a, b By one-way ANOVA and Tukey’s post hoc test. c–e By unpaired Student’s t test, n = 3 per group, * p < 0.05