Fig. 4

Effect of miR-150 regulates the function of early EPCs and late EPCs via the Akt/FOXO1 pathway. (a) Effect of miR-150 on the Akt/FOXO1 pathway, determined by Western blot 72 h after transfection. A representative blot was shown. b Cell surface markers (CD34, VEGFR2, VE-cadherin, CD133, CD31, CD45, CD14, and vWF) were measured in eEPCs with treated miR-150 agomir and wortmannin via flow cytometry. c Tube formation assay showed the change of angiogenetic capacity in the presence of wortmannin. Representative images (upper) and statistical analysis of three independent experiments (lower) are shown. d, e Proliferation capacity and cytokine concentration were tested among different groups (eEPCs, eEPCs+miR-150 agomir, eEPCs+NC agomir, ECFCs and eEPCs+miR-150 agomir+Wortm). (*P < 0.05 vs. eEPCs; **P < 0.01 vs. eEPCs)