Fig. 2

The expression of tsRNAs during hMSC adipogenesis. a tsRNA read distributions during adipogenic differentiation, with tsRNA length variant frequencies given as a percentage of total reads. These identified tsRNAs were primarily 17–23 or 30–36 nucleotides long at all time points. b 5′-tRF halves and tRF-5 sequences had positions similar to those of parental tRNAs, as was true for the majority of 3′-tRF halves and tRF-3 sequences. c The frequencies of tsRNAs during adipogenesis in the overall small RNA-seq, with tsRNA reads provided as a percentage of total reads. The relative frequency of these tsRNAs as a fraction of total non-coding RNAs was significantly increased at all tested time points during adipogenic differentiation. d All six primary classes of tRFs were detectable in these samples, with the relative frequencies of individual tRF subtypes varying over the course of adipogenesis and with the most significant increases being evident on days 7 and 14 of this process