Table 1 Primer sequences used in RT-qPCR
Targets | Primer sequences |
|---|---|
miR-375 | F: 5′-AGCCGTTTGTTCGTTCGGCT-3′ |
R: 5′-GTGCAGGGTCCGAGGT-3′ | |
MELK | F: 5′-CACCGCAGCAGCAGGCAGAC-3′ |
R: 5′-GGGTTGGTGAGGCGGGTATTTC-3′ | |
U6 | F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ |
R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ | |
β-actin | F: 5′-CGTGACATTAAGGAGAAGCTG-3′ |
R: 5′-CTAGAAGCATTTGCGTGGAC-3′ |