Fig. 4

miR-27b overexpression inhibits hypertrophic differentiation of hBMSC-derived chondrocytes. a Expression of COL2, SOX9, RUNX2, CBFB, MMP13, and PPARγ upon overexpression of miR-27b mimic in hBMSCs as measured by RT-qPCR on day 3. *P < 0.05, **P < 0.001. b Western blot measurement of expression of COL2, SOX9, RUNX2, CBFB, MMP13, and PPARγ expression in miR-27b-overexpressing hBMSCs compared to NC on day 3 after transfection. c Semi-quantification of western blot data. *P < 0.05, **P < 0.001. d miR-27b expression on day 0 after miR-27b-LV and miR-27b-SC-LV transfection followed by chondrogenic induction, as measured by RT-qPCR. *P < 0.05. e miR-27b expression on day 14 after miR-27b-LV and miR-27b-SC-LV transfection followed by chondrogenic induction for 14 days, as measured by RT-qPCR. **P < 0.001. f Safranin O staining and IHC for COL2, SOX9, and COL10 in cartilage pellets on day 14. Scale bar = 20 μm. g Safranin O staining was performed to measure GAGs. Optical density for GAGs was evaluated by ImageJ software, and the data represented GAG content. Optical density for COL2, SOX9, and COL10 was evaluated by ImageJ software, and the data represented the expression levels of COL2, SOX9, and COL10. *P < 0.05