Table 1 The BMSC-treated protocol for the flow cytometry analysis of apoptosis
Cell density (cells/well) | H2O2 (μM) procondition/time | LY294002 (μM) pre-incubation/time | H2O2 (μM) treatment/time |
|---|---|---|---|
1 × 105 | No | No | 25, 50, 100, 150, 200, 300, 500/24 h |
1 × 105 | 50 μM/12 h | No | 200, 250, 300, 500/24 h |
1 × 105 | 50 μM/12 h | 50 μM/2 h | 300/24 h |