Fig. 7

hAECs-EXO induced M2 macrophage polarization. a CD206+/F4/80+ M2 macrophage population was measured via flow cytometry. Representative gating strategy was shown. The percentages of M2 macrophages from the total kidney cell population were calculated. b Percentage of M2 Macrophages in kidneys treated with hAECs or hAECs-EXO at day 1, day 2, day 3, and day 7 post-ischemia (n = 3). ^&P < 0.05 vs sham group; ^&&P < 0.01 vs sham group; ^&&&P < 0.001 vs sham group; **P < 0.01 vs IRI+Veh group. ^#P < 0.05 vs IRI+Veh group; ^##P < 0.01 vs IRI+Veh group. c Kidney cytokine concentrations from mice in different groups as indicated at day 1, day2, day 3, and day 7 after IRI (n = 3). *P < 0.05 vs IRI+Veh group; ^#P < 0.05 vs IRI+Veh group; ^##P < 0.01 vs IRI+Veh group. d Bone marrow monocytes were attached for 48 h and collected as control. Bone marrow-derived macrophages were cultured in hAECs-EXO conditioned medium for 7 days and collected. mRNA transcripts of macrophage marker (F4/80) and M1 (Ifnγ, iNos, Tnfα, Cd86) and M2 (Cd163, Cd206, Il4rα, Arg1) markers were determined by qRT-PCR (n = 3). *P < 0.05 vs control group; **P < 0.01 vs control group. Data are shown as mean (SD)