Fig. 1

TESSs from different time points produce pigmented skin after grafting. a Representative H&E-stained images of TESSs at different time points (bars = 50 μm). b Representative images of the skin in graft areas at 4 weeks after transplantation of different TESSs (bars = 5 mm). The white dashed line indicates the border of the host mouse skin and the pigmented human graft skin area. c Representative image of H&E staining of the pigmented area from b. The black dashed line indicates the boundary between the human skin graft area and the host mouse skin. d, e IF staining of human pan-ck (red, d) and human vimentin (green, e) in the pigmented area from b; DAPI stains the nuclei (blue). The white dashed line indicates the boundary between the human skin graft and the host mouse skin (bars = 50 μm). f, g The average size of pigmented skin areas at 4 and 8 weeks after transplantation. *p < 0.05, **p < 0.01 when two groups were compared as indicated; none of the other comparisons was significantly different (3 mice for each group, n = 3)