Fig. 6

Early-stage TESS expresses high levels of p63, but less activation of the apoptotic pathway. a Different stages of TESSs were collected before in vivo grafting for western blot analysis of protein levels of K5, K1, p63, Pro-Caspase-3, and C-Caspase-3. The housekeeping gene GAPDH was used as a loading control. b Quantification of western blot analysis of p63 and C-Caspase-3 relative to the band density of GAPDH. c TESSs as described for a were collected for qRT-PCR analysis of p63, p21, Bax, and Bcl-2. The relative mRNA level of each gene was calculated relative to TESS-5d (expression level as 1) after adjustment by the housekeeping gene 36B4. All experiments were repeated 3 times. *p < 0.05, **p < 0.01, ***p < 0.005 when two groups were compared; none of the other comparisons was significantly different