Fig. 2

MenSCs reduced BLM-induced apoptosis and EMT of MLE-12 cells. a The anti-apoptosis effect of MenSCs on BLM-treated MLE-12 cells examined by CCK8 assay after coculture for 48 h (n = 4/time point). b Clonal formation ability of BLM-treated MLE-12 cells examined by the clonogenic assay (n = 3). Cell apoptosis (c) and cell cycle (d) of BLM-treated MLE-12 cells was performed by flow cytometry (n = 3). e The levels of E-cadherin and N-cadherin protein of BLM-treated MLE-12 cells were measured by IF after cocultured with MenSCs (n = 3). Scale bar: 20 μm. f Migration and invasion of BLM-treated/untreated MLE-12 cells were examined by the wound healing assay and transwell assay after coculture with MenSCs (n = 5). Scale bar: 50 μm. Error bars indicate standard deviation of three biological replicates undergoing independent differentiations. *p < 0.05 and **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way analysis of variance