Fig. 4

FOXQ1 promoted Wnt/β-catenin signaling by interacting with ANXA2. a RT-qPCR of Alp (a), Ocn (b), Opg (c), and Runx2 (d) showed altered Wnt/β-catenin signaling pathway activity with DKK1, and 6BIO reversed the effect of FOXQ1 overexpression and inhibition. b Alizarin red staining of mouse bone mesenchymal stem cells showed altered Wnt/β-catenin signaling pathway activity with DKK1, and 6BIO reversed the effect of changed FOXQ1 expression on osteogenic differentiation. Scale bar 200 μm. c Co-Ip/MS identified 199 proteins bound with FOXQ1 in both control and Foxq1-overexpressing mouse bone mesenchymal stem cells. d Protein-protein interaction network (a) constructed by the STRING online database based on 15 matches, and these proteins were ranked by their coverage percentage (b) in the Co-Ip/MS results. e The TOP/FOP ratio of mouse bone mesenchymal stem cells showed that depletion of Anxa2 mRNA reversed the promotion of FOXQ1 on Wnt/β-catenin signaling. F Schematic representation illustrates FOXQ1 binding to ANXA2, which increases the transnucleation of CTNNB1, promoting Wnt/β-catenin signaling and osteogenesis