Fig. 6

Western blotting of α-SMA, collagen I, fibronectin, and TGF-β1 in UUO kidneys. Compared to the sham-operated control, the obstructed kidneys of vehicle-treated mice showed significantly lower E-cadherin (B) and higher α-SMA (C), collagen I (D), fibronectin (E), and TGF-β1 (F) expression. Treatment with MOCK-MPs, hEPO-MPs, and rhEPO significantly reversed these changes. Protein levels were calculated using NIH Image J software. Western blot data were normalized to GAPDH. Molecular weights are shown in kDa. Data are presented as the mean ± SD; n = 5 for each experimental /group. ^aP < 0.05 vs. sham control, ^bP < 0.05 vs. UUO+vehicle, ^cP < 0.05 vs. UUO+MOCK-MPs, ^dP < 0.05 vs. UUO+hEPO-MPs, ^eP < 0.05 vs. UUO+rhEPO