Fig. 3
From: Acquisition of cancer stem cell properties in osteosarcoma cells by defined factors
Osteogenic differentiation of MG-63 cells cultured in osteogenic induction medium. a Representative images of alkaline phosphatase (ALP) staining and Alizarin Red S staining (inset: macroscopic images). b qRT-PCR of markers related to bone formation (osteocalcin and BMP-2, BMP-4, and BMP-6). The mRNA expression levels were normalized to those of β-actin, and the mRNA expression level of MG-parental cells was set to 1. The error bars indicate the standard error of the mean: SEM. *P < 0.05